A donor film and a method of manufacturing an organic light emitting device using the donor film. The donor film having a stack structure includes: a first layer comprising first transfer portions comprising a light emitting material of a first color, and first opening portions; a second layer that is formed on the first layer and comprising second transfer portions corresponding to the first opening portion and further comprising a light emitting material of a second color, and second opening portions; and a third layer that is formed on the second layer and comprising third transfer portions corresponding to the first and second opening portions and further comprising a light emitting material of a third color, and third opening portions.