A novel maize variety designated PHE64 and seed, plants and plant parts thereof. Methods for producing a maize plant that comprise crossing maize variety PHE64 with another maize plant. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PHE64 through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby. Hybrid maize seed, plant or plant part produced by crossing the variety PHE64 or a trait conversion of PHE64 with another maize variety. Inbred maize varieties derived from maize variety PHE64, methods for producing other inbred maize varieties derived from maize variety PHE64 and the inbred maize varieties and their parts derived by the use of those methods.