A display substrate includes a base substrate, a plurality of a gate line, a gate driving circuit, a starting pad and a first electrostatic dispersion portion. The gate lines are disposed at a display area of the base substrate and extend to the peripheral area. The gate driving circuit is disposed at a peripheral area of the base substrate, includes a plurality of a stage connected to the gate lines, and provides the gate lines with gate signals. The gate driving circuit is driven in response to a vertical starting signal applied to a first stage of the plurality of stages. The starting pad is disposed at the peripheral area and applies the vertical starting signal to the gate driving circuit. The first electrostatic dispersion portion is electrically connected to the starting pad. The first electrostatic dispersion portion disperses electrostatic applied to the gate driving circuit.