A method of transmitting a scheduling request signal, wherein the scheduling request signal is used to request a radio resource for uplink transmission in a wireless communication system is provided. The method includes configuring a physical uplink control channel (PUCCH) for transmission of a scheduling request signal in a subframe, the subframe comprising two consecutive slots, a slot comprising a plurality of single carrier-frequency division multiple access (SC-FDMA) symbols, the scheduling request signal being carried by a presence or absence of transmission of the PUCCH, wherein at least one SC-FDMA symbol is assigned for transmission of a sounding reference signal, and transmitting the scheduling request signal and the sounding reference signal in the subframe. Scheduling request signal can be transmitted without interference with other control signals such as sounding reference signal that are transmitted on the same control channel with the scheduling request signal.