Provided is a fuel cut-off valve capable of preventing liquid fuel from flowing into a valve inside from an evaporation line side, and inhibiting the liquid fuel from flowing into a canister side. The fuel cut-off valve includes: a housing main body including a lower space and an upper space partitioned through a partition wall; a float valve coming into contact with/moving away from a first opening; a lid sealing the upper space; an evaporation line connection pipe communicated to the upper space; a canister connection pipe similarly communicated to the upper space; and a second opening formed in a portion coupled to the evaporation line connection pipe and a third opening formed in a portion coupled to the canister connection pipe, which are surrounded by walls formed in the housing main body and/or the lid, and communicated to each other only through the upper space beyond the walls.