An LCD backlight driving device with an isolating transformer comprises a DC power supply, a square wave generator, a square wave controller, said isolating transformer and a driver transformer; wherein said isolating transformer has a primary side connected to said square wave generator and a secondary side connected to said driver transformer, since said isolating transformer is placed between said square wave generator and said driver transformer, it helps to effectively shorten a safety distance required for setting up said driver transformer; the present invention uses said isolating transformer to shorten the safety distance required than that of using said driver transformer directly and to decrease an area in implementing a circuit board and to cut cost of said device.