An aspect of the present invention provides a wastewater treatment method for treating wastewater containing ammonium nitrogen, including the steps of: oxidizing the ammonium nitrogen in the wastewater to nitrite with nitrifying bacteria in a treatment tank in which the nitrifying bacteria and anaerobic ammonium-oxidizing bacteria having a half-saturation constant of 6.1 mgN/L or more with respect to nitrite coexist; and denitrifying the nitrite formed through the oxidation of the ammonium nitrogen, with the anaerobic ammonium-oxidizing bacteria in the treatment tank, while using the ammonium nitrogen contained in the wastewater as a hydrogen donor. The method employs the anaerobic ammonium-oxidizing bacteria that can maintain the denitrification activity even under a condition in which the amount of dissolved oxygen is large, consequently can easily maintain the activities of both of the nitrifying bacteria and the anaerobic ammonium-oxidizing bacteria which coexist in the same treatment tank, and can rapidly perform wastewater treatment.