An assembly structured to restrict contamination of individuals from association with the heads of stethoscope comprising a housing including a path of travel along which the head portion passes. Different embodiments include the dispensing of cleaning fluid or the delivery of protective barriers onto predetermined portions of the stethoscope heads, while passing along the path of travel. A dispenser assembly and a cooperatively disposed activating assembly are manually operated by moveable, driving engagement with the head portion passing through the housing. The activating assembly is thereby operatively positioned to activate the dispenser assembly when engaged by a head passing along the path of travel. Differently structured dispenser assemblies function to either deliver the cleaning fluid or individually dispense the barriers onto different heads, so as to either facilitate the cleaning or protective covering of exposed surfaces of the stethoscope heads, dependent on the embodiment utilized.