Invention Grant
US08420366B2 Generation of modified polymerases for improved accuracy in single molecule sequencing
有权
修饰聚合酶的生成,提高了单分子测序的准确性
- Patent Title: Generation of modified polymerases for improved accuracy in single molecule sequencing
- Patent Title (中): 修饰聚合酶的生成,提高了单分子测序的准确性
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Application No.: US12924701Application Date: 2010-09-30
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Publication No.: US08420366B2Publication Date: 2013-04-16
- Inventor: Sonya Clark , Arek Bibillo , Paul Peluso , Fred Christians , Molly He , Insil Park , Harold Lee , Keith Bjornson , Lei Jia , Robin Emig
- Applicant: Sonya Clark , Arek Bibillo , Paul Peluso , Fred Christians , Molly He , Insil Park , Harold Lee , Keith Bjornson , Lei Jia , Robin Emig
- Applicant Address: US CA Menlo Park
- Assignee: Pacific Biosciences of California, Inc.
- Current Assignee: Pacific Biosciences of California, Inc.
- Current Assignee Address: US CA Menlo Park
- Agency: Quine Intellectual Property Law Group, P.C.
- Agent Monica Elrod-Erickson; Robert Reamey
- Main IPC: C12N9/12
- IPC: C12N9/12 ; C12P19/34

Abstract:
Provided are compositions comprising recombinant DNA polymerases that include amino acid substitutions, insertions, deletions and/or heterologous or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing. Such properties can include reduced reaction rates at one or more steps of the polymerase kinetic cycle, increased closed polymerase/DNA complex stability, enhanced metal ion coordination, reduced exonuclease activity, decreased branching fractions, and the like. Polymerases that exhibit branching fractions that are less than the branching fractions of the polymerases from which they were derived, or branching fractions that are less than about 25% for a phosphate-labeled nucleotide analog, are also provided. Also provided are nucleic acids which encode the polymerases with the aforementioned phenotypes, as well as methods of using such polymerases to make a DNA or to sequence a DNA template.
Public/Granted literature
- US20110189659A1 Generation of modified polymerases for improved accuracy in single molecule sequencing Public/Granted day:2011-08-04
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