Provided are a transmitter and receiver for a high throughput wireless communication system using a multiple antenna, a method thereof, and a digital intermediate frequency (DIF) transmission signal processing method for the same. The transmitter, includes: a baseband transmitting unit for processing a transmission signal by performing a Media Access Control (MAC) protocol process on transmission data and dividing the transmission data into n band signals where n is a predetermined natural number in a physical layer process; a DIF transmitting unit for integrating transmission signals of each band transmitted from the baseband transmitting unit and outputting m channels where m is a predetermined natural number; and a Radio Frequency (RF) transmitting unit for modulating each channel signal transmitted from the DIF transmitting unit into an RF signal and emitting the signal through the multiple antenna.