A method and multiple antenna transmitting apparatus for transmitting a midamble in a wireless communication system, and a method and receiving device for receiving a midamble in a wireless communication system are provided. A method for transmitting a midamble by a multiple antenna transmitting device includes determining a pattern for a midamble including null subcarriers and pilot subcarriers, wherein each of the pilot subcarriers corresponds to one of a plurality of pilot signals, and each of the plurality of pilot signals corresponds to a different one of a plurality of transmit antennas, and transmitting the pilot signals in corresponding pilot subcarriers based on the determined pattern, wherein the pilot signals are transmitted in an Orthogonal Frequency Division Multiplexing (OFDM) symbol of a resource block comprising a plurality of subcarriers and a plurality of OFDM symbols.