A system and methods for characterizing regions within, or on, an inspected object, wherein a lower-Z scattering material and a higher-Z material may both lie along a common line of sight. The inspected object is scanned with penetrating radiation characterized by an energy distribution, and penetrating radiation scattered by the inspected object is detected in a manner that generates two detector signals that distinguish between materials of higher and lower effective atomic number under distinct sets of conditions with respect to the energy distribution of the penetrating radiation. An image is generated, based on a function of the two detector signals, as is a differential image, so as to allow distinction of higher-Z and lower-Z materials.