Disclosed are a system and a method for generating and regenerating three-dimensional (3D) images file based on two-dimensional (2D) image media standards, a 3D image file generating device for generating a 3D image file having a data area, which includes first image data and second image data synchronized with the first image data so as to be used for generating a 3D image, a header area including information of the first image data, and a metadata area including information of the second image data, and a 3D image file regenerating device, which parses information of the first and the second image data when a 3D image file is inputted so as to synthesize and regenerate the first and the second image data into a 3D image file.