发明授权
US08476484B2 Transgenic clawed frog embryos and use thereof as detectors of endocrine disrupters in the environment
有权
转基因抓青蛙胚胎及其在环境中用作内分泌干扰物的检测器
- 专利标题： Transgenic clawed frog embryos and use thereof as detectors of endocrine disrupters in the environment
- 专利标题（中）： 转基因抓青蛙胚胎及其在环境中用作内分泌干扰物的检测器
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申请号： US10516134申请日： 2003-05-27
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公开(公告)号： US08476484B2公开(公告)日： 2013-07-02
- 发明人: Barbara Demeneix , Nathalie Turque
- 申请人： Barbara Demeneix , Nathalie Turque
- 申请人地址： FR Paris FR Paris
- 专利权人： Centre National de la Recherche Scientifique (CNRS),The Museum National d'Histoire Naturelle
- 当前专利权人： Centre National de la Recherche Scientifique (CNRS),The Museum National d'Histoire Naturelle
- 当前专利权人地址： FR Paris FR Paris
- 代理机构： Foley & Lardner LLP
- 优先权： FR0206669 20020530
- 国际申请： PCT/FR03/01598 WO 20030527
- 国际公布： WO03/102176 WO 20031211
- 主分类号： G01N33/00
- IPC分类号： G01N33/00 ; A01K67/00 ; C12Q1/00
摘要：
The invention relates to transgenic aquatic animals, particularly the clawed frog and the zebra fish and cells derived therefrom, characterized in comprising at least one expression cassette with a regulatory DNA sequence selected from the response elements to nuclear hormone receptors, particularly TRE, connected in a functional manner downstream of a DNA segment coding for a marker protein such as luciferase or GFP. The invention further relates to methods using the transgenic cells and animals according to the invention for the identification of endocrine disrupters in the environment.