A thin film transistor array substrate comprises a transparent substrate, and scanning lines, data lines and pixel regions that are formed on the transparent substrate. Each pixel region comprises a pixel electrode, a thin film transistor for controlling the pixel electrode, a first push alignment electrode and a second push alignment electrode. Projections of the first and second push alignment electrodes on the transparent substrate are located on two opposite sides of the pixel electrode. The pixel electrode is covered with an insulation layer and the insulation layer is provided with a pull alignment opening. When the thin film transistor array substrate is in operation, an oblique pull electric field is formed at the pull alignment opening, and transverse push electric fields are formed between the first push alignment electrode and the pixel electrode as well as between the second push alignment electrode and the pixel electrode, respectively.