发明授权
- 专利标题： Tissue culture system for production of hepatitis C virus
- 专利标题（中）： 组织培养系统用于生产丙型肝炎病毒
-
申请号： US12310309申请日： 2007-08-22
-
公开(公告)号： US08481050B2公开(公告)日： 2013-07-09
- 发明人: Qui-Lim Choo , Jang Han , Michael Houghton , Taewoo Kwon , Hyun Chul Song , Yifei Zhu
- 申请人： Qui-Lim Choo , Jang Han , Michael Houghton , Taewoo Kwon , Hyun Chul Song , Yifei Zhu
- 申请人地址： US CA Emeryville
- 专利权人： Novartis Vaccines and Diagnostics, Inc.
- 当前专利权人： Novartis Vaccines and Diagnostics, Inc.
- 当前专利权人地址： US CA Emeryville
- 代理商 Roberta L. Robins; Kenneth M. Goldman
- 国际申请： PCT/US2007/018591 WO 20070822
- 国际公布： WO2008/024413 WO 20080228
- 主分类号： A61K48/00
- IPC分类号： A61K48/00 ; A61K50/00
摘要：
A tissue culture system for production of infectious hepatitis C virus is described. In particular, the invention provides recombinant monocistronic and bicistronic genomic constructs for production of virus, including constructs for production of wild-type HCV type 2a strain JFH1 and constructs for production of chimeric viruses comprising HCV proteins from strain JFH1 and a second HCV isolate. Constructs of the invention also include a reporter gene to facilitate measurement of RNA replication and viral infectivity in cultures. The cell culture system may also include various factors that improve viral replication or infectivity. In addition, a neutralization assay using HCV grown in cell culture is described.