The present invention provides a light source assembly, which comprises a light tube, a conductive nesting element, a first insulative insert and a second insulative insert. A nesting body of the conductive nesting element is fitted over and fixed to an end of a tube body of the light tube and is electrically connected to a pin of the light tube, and a flap of the conductive nesting element is disposed on the nesting body and extends in a direction lateral to the light tube. The first insulative insert is formed with a first through-hole and a blind hole. The nesting body is inserted through the first through-hole, and the flap is disposed and supported in the blind hole from a side away from the light tube. The second insulative insert comprises an insulative body and a conductive elastic piece disposed inside the insulative body. Both ends of the conductive elastic piece are exposed from two opposite sides of the insulative body, and one end of the conductive elastic piece abuts against the flap located in the blind hole. The present invention further provides a liquid crystal display (LCD). With the above arrangement, damage to the light tube is prevented when a lead is welded onto the pin of the light tube, so the light source assembly and the LCD of the present invention can save the cost.