An adjusting method for a lens unit used in an image reading apparatus which images image information of an original onto an image reading unit by the lens unit and reads the image information, the lens unit including rotationally-symmetrical lenses, a lens barrel including the rotationally-symmetrical lenses and an adjusting lens, the adjusting method including: performing rotational adjustment of the lens barrel with respect to the adjusting lens; and imaging an adjusted chart onto one-dimensional photoelectric transducers via the lens unit, obtaining contrast depth characteristics of images corresponding to at least three angles of field of the lens unit among images of the adjusted chart, and, according to the obtained contrast depth characteristics, performing position adjustment of the adjusting lens in at least one of an array direction of the one-dimensional photoelectric transducers, a direction orthogonal to the array direction and an optical axis direction of the lens unit.