The present disclosure is directed at methods and apparatus to evaluate and monitor healing progress of wound and/or to generate data to modify or identify a treatment protocol. Fluid exudate removed from the wound by a negative pressure therapy device may now be analyzed to identify the progress of wound healing, such as whether healing is progressing in a positive manner or experiencing one or more impediments. The fluid exudates may be specifically analyzed for one or more analytes indicative of one or more of the biochemical reactions that may occur during wound recovery. In addition, one may separately utilize optical sensors integrated into a dressing enclosure, optionally in those dressings employed in a negative pressure therapy device. Such optical sensors may then illuminate the wound and collect information regarding the wound via a light scattering type response.