A calibration apparatus and a calibration method for multicell Multiple Input Multiple Output (MIMO) transmission in a multiple antenna system are provided. More particularly, an operating method of a Mobile Station (MS) for performing calibration for multicell MIMO transmission in a multiple antenna system includes negotiating a multi-Base Station (BS) joint processing with a BS, receiving a control message including information instructing multi-BS calibration for sounding based on the multi-BS joint processing, from the BS; when receiving the control message involving the multi-BS joint processing, generating a second sounding sequence by mapping estimated phases of downlink channels per subcarrier of BSs to a first sounding sequence allocated from the BS, and transmitting the second sounding sequence, including the phases of the downlink channels per subcarrier, over a sounding symbol interval with respect to each of the BSs involving the multi-BS joint processing. Hence, feedback overhead in the calibration of the multicell MIMO transmission can be reduced.