A yeast biofilm microbial fuel cell has anode and cathode chambers, each containing an electrolyte medium, separated by a proton conducting membrane. A baker's yeast biofilm is induced to form on the anode under electrical poising. A method of making the MFC includes adding baker's yeast and yeast nutrient fuel source to the anode solution, connecting a resistor across the anode and cathode to enable current flow through the resistor for a selected time for poising the anode and formation of the anodic yeast biofilm, replacing the anode solution with a fresh quantity of yeast-free solution, adding fuel source to the solution, and continuing to run the MFC for a selected time under resistance. The steps of replacing the anode solution, adding fuel source and running the cell under load are repeated until the baker's yeast has formed a suitable anodic biofilm.