A method of transmitting an uplink reference signal in a multiple antenna system is provided. A user equipment transmits a first sounding reference signal for uplink channel measurement on a first antenna by using at least one power amplifier among a plurality of power amplifiers, and transmits a second sounding reference signal for uplink channel measurement on a second antenna by using the at least one power amplifier, wherein the first sounding reference signal and the second sounding reference signal are transmitted using different time regions when using one power amplifier, and the first sounding reference signal and the second sounding reference signal are transmitted using different time regions or different frequency regions when using the plurality of power amplifiers.