The present invention is directed towards systems and methods for dynamically deploying and executing acceleration functionality on a client to improve the performance and delivery of remotely accessed applications. In one embodiment, the client-side acceleration functionality is provided by an acceleration program that performs a transport layer connection multiplexing technique for improving performance of communications and delivery of a remotely-accessed application. The acceleration program establishes a transport layer connection from the client to the server that can be used by multiple applications on the client, or that is otherwise shared among applications of the client. The acceleration program multiplexes requests from one or more applications via the same transport layer connection maintained by the acceleration program. As such, the acceleration program can utilize the idle time of one application in using the transport layer connection to service a request of a second application through the same transport layer connection.