The present invention relates to a cell-permeable endostatin recombinant protein in which a macromolecule transduction domain (MTD) is fused to an angiogenesis inhibitor (angiogenesis inhibitor) endostatin; a polynucleotide encoding the cell-permeable endostatin recombinant protein; an expression vector for the cell-permeable endostatin recombinant protein; and a pharmacological composition for an anti-cancer preparation with improved inhibitory activity against angiogenesis in cancer, which contains the cell-permeable endostatin recombinant protein as an active component. The cell-permeable endostatin recombinant protein according to the present invention can block the formation of microvessels and inhibit the migration, proliferation, penetration, tube formation and the like of vascular endothelial cells present in tumor tissue by introducing the angiogenesis inhibitor endostatin into the cell with high efficiency, and it exhibits outstanding anti-cancer activity and so can be used as an anti-cancer drug against various cancers.