A method for integrating data deduplication with block level incremental data backup is described. In one embodiment, the method includes accessing from memory at least one modified data block of a file, wherein the file is partitioned into a plurality of deduplication segments, identifying at least one deduplication segment that corresponds with the at least one modified data block, computing using at least one processor a signature for each deduplication segment of the at least one corresponding deduplication segment and comparing using at least one processor each signature that is associated with the at least one corresponding deduplication segment with signature information that is associated with backup data to identify at least one duplicate data block within the backup data and the at least one modified data block.