发明授权
US08658356B2 Methods and compositions for the specific inhibition of gene expression by double-stranded RNA
有权
通过双链RNA特异性抑制基因表达的方法和组合物
- 专利标题： Methods and compositions for the specific inhibition of gene expression by double-stranded RNA
- 专利标题（中）： 通过双链RNA特异性抑制基因表达的方法和组合物
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申请号： US13152686申请日： 2011-06-03
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公开(公告)号： US08658356B2公开(公告)日： 2014-02-25
- 发明人: John J. Rossi , Mark A. Behlke , Dongho Kim
- 申请人： John J. Rossi , Mark A. Behlke , Dongho Kim
- 申请人地址： US CA Duarte US IA Coralville
- 专利权人： City of Hope,Integrated DNA Technologies, Inc.
- 当前专利权人： City of Hope,Integrated DNA Technologies, Inc.
- 当前专利权人地址： US CA Duarte US IA Coralville
- 代理机构： Rothwell, Figg, Ernst & Manbeck P.C.
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68
摘要：
The invention is directed to compositions and methods for selectively reducing the expression of a gene product from a desired target gene in a cell, as well as for treating diseases caused by the expression of the gene. More particularly, the invention is directed to compositions that contain double stranded RNA (“dsRNA”), and methods for preparing them, that are capable of reducing the expression of target genes in eukaryotic cells. The dsRNA has a first oligonucleotide sequence that is between 25 and about 30 nucleotides in length and a second oligonucleotide sequence that anneals to the first sequence under biological conditions. In addition, a region of one of the sequences of the dsRNA having a sequence length of at least 19 nucleotides is sufficiently complementary to a nucleotide sequence of the RNA produced from the target gene to trigger the destruction of the target RNA by the RNAi machinery.