The present invention provides a tube clamping and closing device which includes a main body having a first end and a second end, and a retaining assembly having several kinds of work portions and disposed at the second end of the main body. The first end has a first clamping member extending laterally and having a threaded hole extending vertically. A blocking member is threadedly connected to the threaded hole and adapted for closing an opening of a tube. The work portions alternatively define a clamping space with the first clamping member and the main body. Whereby a single device of the present invention can be used for different kinds of tubes or multiport valves. The present invention has advantages of operating conveniently, carrying and putting away easily, saving space and costs, etc.