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US08685721B2 Stable constitutively high expression vector for preparing HPV vaccine and recombinant lactic acid bacteria transformed thereby 有权
用于制备HPV疫苗和由此转化的重组乳酸菌的稳定的组成型高表达载体

Stable constitutively high expression vector for preparing HPV vaccine and recombinant lactic acid bacteria transformed thereby
Abstract:
A surface expression vector for preparing HPV immunogenic compositions, in which the surface expression vector contains a gene encoding a repE mutant protein having an amino acid sequence of SEQ ID NO: 1, a promoter, a poly-gamma-glutamate synthetase complex gene, and a gene which is linked with the poly-gamma-glutamate synthetase complex gene and encodes a tumor induction-associated antigen protein of human papillomavirus.An expression vector constitutively expressing a high level of the human papillomavirus (HPV) antigen protein is provided. Also, a recombinant lactic acid bacteria, transformed with the expression vector and expressing the HPV antigen protein on the surface thereof, and a composition comprising the recombinant lactic acid bacteria are provided. The recombinant lactic acid bacteria and the composition are very effective as a immunogenic composition for the treatment of cervical cancer, because they can be applied orally or directly to the vagina.
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