Invention Grant
US08685721B2 Stable constitutively high expression vector for preparing HPV vaccine and recombinant lactic acid bacteria transformed thereby
有权
用于制备HPV疫苗和由此转化的重组乳酸菌的稳定的组成型高表达载体
- Patent Title: Stable constitutively high expression vector for preparing HPV vaccine and recombinant lactic acid bacteria transformed thereby
- Patent Title (中): 用于制备HPV疫苗和由此转化的重组乳酸菌的稳定的组成型高表达载体
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Application No.: US13143700Application Date: 2010-01-08
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Publication No.: US08685721B2Publication Date: 2014-04-01
- Inventor: Moon-Hee Sung , Haryoung Poo , Il Han Lee
- Applicant: Moon-Hee Sung , Haryoung Poo , Il Han Lee
- Applicant Address: KR Daejeon KR Seoul
- Assignee: Bioleaders Corporation,Kookmin University Industry-Academy Cooperation Foundation
- Current Assignee: Bioleaders Corporation,Kookmin University Industry-Academy Cooperation Foundation
- Current Assignee Address: KR Daejeon KR Seoul
- Agency: Rabin & Berdo, P.C.
- Priority: KR10-2009-0001510 20090108
- International Application: PCT/KR2010/000126 WO 20100108
- International Announcement: WO2010/079991 WO 20100715
- Main IPC: C12N15/37
- IPC: C12N15/37 ; A61K39/12 ; A61K39/07 ; A61K39/09

Abstract:
A surface expression vector for preparing HPV immunogenic compositions, in which the surface expression vector contains a gene encoding a repE mutant protein having an amino acid sequence of SEQ ID NO: 1, a promoter, a poly-gamma-glutamate synthetase complex gene, and a gene which is linked with the poly-gamma-glutamate synthetase complex gene and encodes a tumor induction-associated antigen protein of human papillomavirus.An expression vector constitutively expressing a high level of the human papillomavirus (HPV) antigen protein is provided. Also, a recombinant lactic acid bacteria, transformed with the expression vector and expressing the HPV antigen protein on the surface thereof, and a composition comprising the recombinant lactic acid bacteria are provided. The recombinant lactic acid bacteria and the composition are very effective as a immunogenic composition for the treatment of cervical cancer, because they can be applied orally or directly to the vagina.
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