An inter-cylinder air-fuel ratio imbalance abnormality determination device includes a catalyst provided in an exhaust passage of a multi-cylinder internal combustion engine; a pre-catalyst sensor; a post-catalyst sensor; an air-fuel ratio control unit that performs main air-fuel ratio control based on an output of the pre-catalyst sensor and auxiliary air-fuel ratio control based on an output of the post-catalyst sensor; a control amount calculation unit that calculates a control amount in the auxiliary air-fuel ratio control based on the output of the post-catalyst sensor; a revolution speed variation detection unit that detects a revolution speed variation of the engine; an abnormality determination unit that performs imbalance abnormality determination for determining whether an inter-cylinder air-fuel ratio imbalance abnormality has occurred based on a detected value of the revolution speed variation; and a guard range reduction unit that reduces a guard range of the control amount during the imbalance abnormality determination.