发明授权
- 专利标题: Microfluidic free interface diffusion techniques
- 专利标题(中): 微流体界面扩散技术
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申请号: US13213990申请日: 2011-08-19
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公开(公告)号: US08709152B2公开(公告)日: 2014-04-29
- 发明人: Carl L. Hansen , Stephen R. Quake , James M. Berger
- 申请人: Carl L. Hansen , Stephen R. Quake , James M. Berger
- 申请人地址: US CA Padadena US CA Oakland
- 专利权人: California Institute of Technology,The Regents of the University of California
- 当前专利权人: California Institute of Technology,The Regents of the University of California
- 当前专利权人地址: US CA Padadena US CA Oakland
- 代理机构: Kilpatrick Townsend & Stockton LLP
- 主分类号: C30B7/14
- IPC分类号: C30B7/14
摘要:
A static fluid and a second fluid are placed into contact along a microfluidic free interface and allowed to mix by diffusion without convective flow across the interface. In accordance with one embodiment of the present invention, the fluids are static and initially positioned on either side of a closed valve structure in a microfluidic channel having a width that is tightly constrained in at least one dimension. The valve is then opened, and no-slip layers at the sides of the microfluidic channel suppress convective mixing between the two fluids along the resulting interface. Applications for microfluidic free interfaces in accordance with embodiments of the present invention include, but are not limited to, protein crystallization studies, protein solubility studies, determination of properties of fluidics systems, and a variety of biological assays such as diffusive immunoassays, substrate turnover assays, and competitive binding assays.
公开/授权文献
- US20120046639A1 MICROFLUIDIC FREE INTERFACE DIFFUSION TECHNIQUES 公开/授权日:2012-02-23