发明授权
- 专利标题: Reversible di-nucleotide terminator sequencing
- 专利标题(中): 可逆二核苷酸终止子测序
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申请号: US13198901申请日: 2011-08-05
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公开(公告)号: US08715938B2公开(公告)日: 2014-05-06
- 发明人: Gerald Zon
- 申请人: Gerald Zon
- 申请人地址: US CA Carlsbad
- 专利权人: Life Technologies Corporation
- 当前专利权人: Life Technologies Corporation
- 当前专利权人地址: US CA Carlsbad
- 主分类号: C12Q1/68
- IPC分类号: C12Q1/68 ; C12P19/34 ; C07H21/04
摘要:
The present teachings provide methods, compositions, and kits for synthesizing and sequencing nucleic acids. In some embodiments, reversible di-nucleotide compounds are employed along with cleaving reactions that remove a label and a blocking moiety. Improved sequencing efficiency is achieved by the rapid polymerase-mediated incorporation of reversible di-nucleotide compounds. In some embodiments, the di-nucleotides do not contain conventional nucleotide triphosphates, but rather employ amino acid phosphoramidate nucleotides (AAPNs).
公开/授权文献
- US20130065225A1 Reversible Di-Nucleotide Terminator Sequencing 公开/授权日:2013-03-14