发明授权
US08741595B2 Coliform detection process and kit for use therein 有权
大肠杆菌检测过程及其中使用的试剂盒

  • 专利标题: Coliform detection process and kit for use therein
  • 专利标题(中): 大肠杆菌检测过程及其中使用的试剂盒
  • 申请号: US13142455
    申请日: 2009-12-29
  • 公开(公告)号: US08741595B2
    公开(公告)日: 2014-06-03
  • 发明人: Manjiri T. Kshirsagar
  • 申请人: Manjiri T. Kshirsagar
  • 申请人地址: US MN St. Paul
  • 专利权人: 3M Innovative Properties Company
  • 当前专利权人: 3M Innovative Properties Company
  • 当前专利权人地址: US MN St. Paul
  • 代理商 Adrian L. Pishko; Stephen L. Crooks
  • 国际申请: PCT/US2009/069629 WO 20091229
  • 国际公布: WO2010/078284 WO 20100708
  • 主分类号: C12Q1/04
  • IPC分类号: C12Q1/04
Coliform detection process and kit for use therein
摘要:
A process comprises (a) providing (1) at least one sample suspected of comprising at least one coliform strain, (2) at least one culture device comprising at least one culture medium that is hydrated or hydratable, and (3) at least one particulate concentration agent that is substantially optically transparent when in contact with the culture medium in the culture device when the culture medium is hydrated; (b) placing the particulate concentration agent in contact with the sample such that at least a portion of the coliform strain is bound to the particulate concentration agent to form coliform-bound particulate concentration agent; (c) placing the coliform-bound particulate concentration agent in contact with the culture medium; (d) incubating the culture device comprising the coliform-bound particulate concentration agent in contact with the culture medium, the culture medium being hydrated; and (e) optically detecting the presence of the coliform strain without separating the coliform strain from the particulate concentration agent.
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