An operational amplifier with different power supply voltages includes an input stage and an output stage. The input stage includes a current source for providing a bias current, and a differential input circuit for receiving the bias current and differential input voltage signals, and converting the differential input voltage signal to differential input currents. The input stage is supplied by a first power supply voltage. The output stage includes a load circuit coupled to the differential input voltage signal and for receiving the differential input currents, and outputting a single ended output voltage signal. The output stage is supplied by a second power supply voltage. The second power supply voltage is lower than the first power supply voltage.