A fundus examination device aiding in gaze fixation and image focusing includes a light projecting device for projecting an examination light to illuminate an examinee's fundus; an illuminating system for transmitting the examination light to the examinee's eye and receiving a fundus image; an imaging system for showing the fundus image; and a focusing and gaze-fixation device located in the illuminating system and including a focus mask formed in a focusing zone, on which the examinee's eye focuses. The focus mask includes a split image screen surrounded by a light-penetrable structure, and gaze fixation devices for forming gaze-fixation images at examinee's eye focusing positions within the focusing zone, such that the split image screen and the gaze-fixation images are located at different focal positions corresponding to the examinee's eye curvature. Therefore, when a split image focusing is completed, the gaze-fixation images are also located at clearly recognizable focal positions.