Provided are a receiver for a real time locating system (RTLS) and a method of receiving a signal in the RTLS. The receiver includes a down converter converting a radio frequency (RF) signal into a base band signal, a matched filter filtering the converted signal using an optimal receiving filter in view of a signal-to-noise ratio (SNR) such as a shaping filter used for a transmitter, an adaptive transversal filter (ATF) estimating an interference signal with an output of the matched filter, and filtering the output to remove interference of a heterogeneous signal of the same frequency band, a de-spreader multiplying an output of the matched filter and a code according to DSSS modulation used for spreading when a tag signal is modulated, and performing chip-matched filtering to obtain a value of correlation energy, and a detector detecting original transmission data using the correlation energy value.