The embodiments of the present invention disclose resource configuration methods and devices of a demodulation reference signal. The method includes determining the first number of a first transmission layers of a user equipment (UE) for transmitting uplink data; selecting a combination of a Cyclic Shift (CS) resource and an Orthogonal Cover Code (OCC) sequence from one combination item according to the first number of the first transmission layers for each of the first transmission layers; wherein the one combination item is among a plurality of predetermined combination items, each of the plurality of predetermined combination items comprises the second number of predetermined CS resources and OCC sequences respectively corresponding to the maximum number of transmission layers supportable by the communication system. The solutions of the present invention can simplify the complexity of a protocol describing the DMRS resource configurations, and is helpful for reducing inter-codeword DMRS interferences.