A network analysis system invokes an application specific, or source-destination specific, path discovery process. The application specific path discovery process determines the path(s) used by the application, collects performance data from the nodes along the path, and communicates this performance data to the network analysis system for subsequent performance analysis. The system may also maintain a database of prior network configurations to facilitate the identification of nodes that are off the path that may affect the current performance of the application. The system may also be specifically controlled so as to identify the path between any pair of specified nodes, and to optionally collect performance data associated with the path.