发明授权
- 专利标题: Chip-based sequencing nucleic acids
- 专利标题(中): 基于芯片的测序核酸
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申请号: US11965585申请日: 2007-12-27
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公开(公告)号: US08815576B2公开(公告)日: 2014-08-26
- 发明人: Neil Reginald Beer
- 申请人: Neil Reginald Beer
- 申请人地址: US CA Livermore
- 专利权人: Lawrence Livermore National Security, LLC.
- 当前专利权人: Lawrence Livermore National Security, LLC.
- 当前专利权人地址: US CA Livermore
- 代理商 Eddie E. Scott
- 主分类号: C12Q1/68
- IPC分类号: C12Q1/68 ; C12M1/00 ; C12M3/00 ; C12M1/34
摘要:
A system for fast DNA sequencing by amplification of genetic material within microreactors, denaturing, demulsifying, and then sequencing the material, while retaining it in a PCR/sequencing zone by a magnetic field. One embodiment includes sequencing nucleic acids on a microchip that includes a microchannel flow channel in the microchip. The nucleic acids are isolated and hybridized to magnetic nanoparticles or to magnetic polystyrene-coated beads. Microreactor droplets are formed in the microchannel flow channel. The microreactor droplets containing the nucleic acids and the magnetic nanoparticles are retained in a magnetic trap in the microchannel flow channel and sequenced.
公开/授权文献
- US20100184020A1 Chip-Based Sequencing Nucleic Acids 公开/授权日:2010-07-22
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