Tissue array production method enables even roll-shaped tissue pieces having various diameters to be steadily fixed to a substrate block is provided. In a method in which roll-shaped tissue pieces formed by rolling sheet-like tissue pieces in the shape of a roll are arranged on a substrate in an array form, a tissue array is produced by placing a holding member which holds a plurality of roll-shaped tissue pieces so that their axis directions are vertically directed in a container into which a melted embedding medium is poured for its accumulation, to hold the respective roll-shaped tissue pieces in the holding member; pouring the embedding medium into the container, to form a substrate block constituted so that the plurality of roll-shaped tissue pieces is arranged in the array form; and slicing the substrate block so that the roll-shaped tissue pieces are ring-shaped.