A reverse opening slide fastener is provided with a pair of first and second separable connection members which are firmly fixed to first and second fastener stringers, and a pair of first and second sliders. A connection and separation operation can be carried out by inserting and removing the second separable connection member into and from the first and second sliders. The second separable connection member has an inserting and removing region which can be inserted into and removed from the first and second sliders via a gap between upper and lower flanges of the first and second sliders. Further, a positioning structure which decides a relative position between the first separable connection member and the second separable connection member is arranged, whereby it is possible to improve an operability of the connection and separation operation.