A tamper detector is provided for safeguarding a radiological source that is moved into and out of a storage location through an access porthole for storage and use. The radiological source is presumed to have an associated shipping container approved by the U.S. Nuclear Regulatory Commission for transporting the radiological source. The tamper detector typically includes a network of sealed tubing that spans at least a portion of the access porthole. There is an opening in the network of sealed tubing that is large enough for passage therethrough of the radiological source and small enough to prevent passage therethrough of the associated shipping cask. Generally a gas source connector is provided for establishing a gas pressure in the network of sealed tubing, and a pressure drop sensor is provided for detecting a drop in the gas pressure below a preset value.