In a light-guide module, a method of manufacturing the light-guide module and a backlight assembly having the light-guide module, the light-guide module includes a light-guide plate (“LGP”) and a thin-film layer. The LGP has a light-incident surface into which lights are incident and a light-exiting surface through which lights exit. The thin-film layer is formed on the LGP. The thin-film layer has a concavo-convex pattern formed on an opposite surface of a surface contacting the LGP. Accordingly, a thin-film layer having a concavo-convex pattern is formed on a light-incident surface of an LGP, so that a reflectance of light incident into the LGP may be decreased. Moreover, a light amount transmitted through the LGP is increased in accordance with a decreasing of reflectance, so that a light transmittance may be increased in total.