An electrochemical test strip is provided, including a substrate, an electrode structure and an insulative film. The electrode structure is formed on the substrate, including a first electrode and a second electrode. The second electrode includes a first end, a second end, an extension portion, and a bent portion. The extension portion connects the first end with the bent portion, and the bent portion is connected to the second end. The extension portion and the first electrode define a space therebetween for receiving the bent portion. The insulative film covers at least a part of the electrode structure and forms an opening. A sample fluid enters the electrochemical test strip through the opening, and the sample fluid sequentially contacts the first electrode and the second electrode.