An overcurrent protection device includes a power input terminal, a power output terminal, a first signal terminal, a second signal terminal, a testing circuit, and a switch element. The power input terminal and the first signal terminal are connected to a power supply. The power output terminal and the second signal terminal are connected to a computer motherboard. If the first and second terminals are disconnected from each other when the computer motherboard works, the power supply stops working. The testing circuit includes a fixed resistor and a control chip parallel connected between the power input and output terminal. The control chip stores a predetermined voltage threshold, and detects voltage between the two terminals of the fixed resistor, and compares the measured voltage with the predetermined voltage threshold. The switch element disconnects the first and second signal terminals when the measured voltage is greater than the predetermined voltage threshold.