Methods, systems, and computer readable media utilizing metadata to detect UDP traffic loss in a test environment are disclosed. In one example, the method includes generating, at a user datagram protocol (UDP) traffic generation module, a plurality of UDP packets associated with a UDP stream, wherein origination information corresponding to the UDP traffic generation module is included in a payload in each of the plurality of UDP packets and receiving, at a UDP traffic receiver module, at least one of the plurality of UDP packets associated with the UDP stream. The method also includes transmitting, from the UDP traffic receiver module to the UDP traffic generation module, recipient information that identifies the UDP traffic receiver module as the receiver of the UDP stream and receiving, at the UDP traffic receiver module via a lossless environment, stream verification metrics from the UDP traffic generation module.