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发明授权
US08889359B2 Method and kit for amplifying and detecting polynucleotide using a modified Tth polymerase 有权
使用修饰的第T聚合酶扩增和检测多核苷酸的方法和试剂盒

Method and kit for amplifying and detecting polynucleotide using a modified Tth polymerase
摘要：
Disclosed is a method of amplifying a polynucleotide, comprising: (a) mixing primers for amplifying the polynucleotide, a polymerase, nucleotide substrates and a template polynucleotide, and (b) amplifying the polynucleotide by polymerase reaction, wherein the polymerase has an amino acid sequence consisting of SEQ ID NO: 1 or an amino acid sequence with at least 85% sequence identity to SEQ ID NO: 1, and wherein an amino acid residue corresponding to position 653 of the amino acid sequence has been replaced with glutamic acid.
摘要（中）：

公开了扩增多核苷酸的方法，包括：（a）混合用于扩增多核苷酸的引物，聚合酶，核苷酸底物和模板多核苷酸，和（b）通过聚合酶反应扩增多核苷酸，其中所述聚合酶具有氨基酸序列 由SEQ ID NO：1或与SEQ ID NO：1具有至少85％序列同一性的氨基酸序列组成，其中对应于氨基酸序列的位置653的氨基酸残基已被谷氨酸替代。

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