An organic light-emitting substrate includes a base substrate, a gate line, a data line, a bias line, an organic light-emitting diode, a switching transistor, a driving transistor and a repair line. The bias line is spaced apart from the gate line and the data line. The organic light-emitting diode includes a pixel electrode, a common electrode and an organic light-emitting part. The switching transistor is connected to the gate line and the data line. The driving transistor is connected to the bias line, the pixel electrode and the switching transistor. The repair line is formed from a pixel metal layer that is identical to the pixel electrode to be spaced apart from the pixel electrode, and is formed along the first direction to be overlapped with the gate line. Therefore, the repair line may repair electric defects of the gate line.