Provided is a Multiple Input Multiple Output (MIMO) transmission method in a digital broadcasting system. The method includes generating a plurality of first modulation symbols by modulating first information bits; generating a plurality of second modulation symbols by modulating second information bits; generating a plurality of first pre-coded symbols and a plurality of second pre-coded symbols by pre-coding the plurality of first modulation symbols and the plurality of second modulation symbols; and allocating the plurality of first pre-coded symbols and the plurality of second pre-coded symbols staggeringly through a first antenna and a second antenna and transmitting them.