What is disclosed is a video-based system and method for estimating heart rate variability from time-series signals generated from video images captured of a subject of interest being monitored for cardiac function. In a manner more fully disclosed herein, low frequency and high frequency components are extracted from a time-series signal obtained by processing a video of the subject being monitored. A ratio of the low and high frequency of the integrated power spectrum within these components is computed. Analysis of the dynamics of this ratio over time is used to estimate heart rate variability. The teachings hereof can be used in a continuous monitoring mode with a relatively high degree of measurement accuracy and find their uses in a variety of diverse applications such as, for instance, emergency rooms, cardiac intensive care units, neonatal intensive care units, and various telemedicine applications.